FastQCFastQC Report
Thu 21 Sep 2017
H2FN2BGX3_n01_meseq_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FN2BGX3_n01_meseq_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26693728
Sequences flagged as poor quality0
Sequence length75
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCT21150.055.60613
AAGCCTA41400.054.8989834
AGCCTAA41000.054.5091675
GCCTAAG41950.051.5482526
CCTAAGT42350.051.055357
CCCTGGC1150.051.007751
AAAGCCT21550.050.2528573
CCTGGGT15200.050.1688081
ACATAGC20100.048.9253461
AAAAGCC22300.048.408262
GGCGCAC5250.047.95867568
CCTAGGC3600.047.923951
GTAAGCC10850.047.044842
CTAAGTA225550.046.983848
CCTGGGG6950.046.1724851
CATAGCG22300.046.088382
AAGACAC66250.045.8461830
AGACACA67250.045.32029331
CCTTGTT25000.045.24476221
ATCCTTG11900.044.9177119
Produced by FastQC (version 0.11.2)