FastQCFastQC Report
Thu 21 Sep 2017
H2FN2BGX3_n01_meseq_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FN2BGX3_n01_meseq_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20943473
Sequences flagged as poor quality0
Sequence length75
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG302060.14422631814694725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCTAA31150.052.481545
AAGCCTA31050.052.3172074
TAAGCCT15300.052.297353
AAAGCCT16400.049.8411063
ACATAGC15350.049.2270161
GCCTAAG34400.047.9244086
CCTAAGT33750.047.921277
CCTGGGT12550.047.0132371
CATAGCG16300.046.7616582
GCGCACC5400.046.62540469
GGCGCAC5350.046.41648568
AAGACAC48650.045.61786730
CCTAGGC2600.045.120441
CTAAGTA163600.045.0662658
CCCGTAC1400.044.3621141
AGACACA50400.044.10407631
AAAAGCC18850.043.729222
GACACAA50950.043.697532
GTATCCT9350.043.5213917
CCCCCTT1050.042.7190741
Produced by FastQC (version 0.11.2)