FastQCFastQC Report
Thu 21 Sep 2017
H2FN2BGX3_n01_lblrna_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FN2BGX3_n01_lblrna_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19141777
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC3224121.6843368303789141No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTA2598581.3575437640925396No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC2328581.2164910290199287No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATC1856770.9700092107436004No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCT1748370.9133791496996333No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTAC1449620.7573069104294757No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCTAACCTCTGTAGGAC1180060.6164840390732794No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTA889420.4646486060306731No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGT864070.4514053214599668No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG805850.4209901724380134TruSeq Adapter, Index 11 (100% over 63bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTGTTTGATC728470.3805655034012777No Hit
ATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAA699820.3655982409574618No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCTAACCTCT663140.3464359656890789No Hit
TGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTAAAAATTCTCGTG637870.3332344745213571No Hit
TCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAAT635240.33186051639824243No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCT632870.3306223868348273No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCTA631910.3301208659990136No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACTAGGTTACTCTGTAGGGTAAAAATTCT608010.317635086857401No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCTAACCTCTGTAGGACT553970.2894036431413865No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCT541520.28289954480192725No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACT540230.28222562617880254No Hit
CAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTAAAAAT538920.28154125920493167No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC522620.2730258533468444No Hit
GTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGG499690.26104681921641865No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCA463420.24209873513833122No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTG455690.2380604475749561No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACTAGGTTA445990.2329929974630882No Hit
GTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTAA437060.2283278088549459No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACTAGGT417950.2183444097170289No Hit
TGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTA417260.21798394161628779No Hit
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCTAA400490.2092229995156667No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACTAGGTTAC353200.18451787417646753No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG337350.17623755621016796TruSeq Adapter, Index 11 (100% over 63bp)
AGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCTAACCTC319160.16673478120657242No Hit
GTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTAAAAATTCTCGTGTTCCG317860.16605563840807464No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAATCTAGGTTA288430.15068089028516005No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACTAGGTTACTC287270.15007488594188512No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAATCTAGGT282820.14775012790087358No Hit
GTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGG281300.14695605324416852No Hit
CGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCTAACCTCTGTAGGACTATCAGG281110.14685679391208037No Hit
TACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCTAACCTCTG274270.14328345795690756No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAATCTAGGTTACTCTGTAGGGTAAAAATTCT263750.13778762546444878No Hit
TTTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC262150.13695175740475923No Hit
AAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCTAACCTCTGTAG261460.13659128930401812No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGA260560.13612111352044276No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACTAGGTTACTCTGTAGGGTAAAAATTCTC257150.13433966971822942No Hit
TAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGT254580.13299705664735306No Hit
CCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTAAAAATTCTCG248800.1299774832817246No Hit
GAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCTAACCT241240.12602800669969147No Hit
AACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTAAAA239440.12508765513254072No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACAT233500.12198449496094328No Hit
AGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCTAACC225290.11769544697966129No Hit
ATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTT225100.11759618764757315No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAATCTAGGTTAC220650.1152714296065616No Hit
GTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTAAAAATTCTCGTGTTCCGTTTGTACTCTA220190.11503111753940086No Hit
TTTTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG214650.1121369243827258No Hit
GTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTG211730.11061146517379239No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACTAGGTTACTCTGTA199150.10403945255448331No Hit
CTACTATCTAACCTCTGTAGGACTATCAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTAAAAATTCTCGT197780.10332374052837415No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG195740.10225800875226998No Hit
CAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTAAAAATTCTCGTGTTCCGTTTGTA195510.1021378527186896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG252950.056.393725
CTCTAGT261050.054.472016
TTTTTCC831050.050.000851
TCTAGTT298250.047.7763067
TTTTCCA899750.046.435552
TGATCCA460750.046.2799641
GTATGCC159100.043.46157545
CGTATGC159200.043.45459444
TATGCCG160350.043.16571846
CTAGTTG332500.042.378018
GATCCAA510350.042.0008352
TCGTATG165150.041.92643
TAGTTGT338550.041.6851969
ATGCCGT169050.041.08717747
CGGCTAC168950.040.96214733
CATCTCG170750.039.20879739
GGCTACA175050.039.18011534
GCCGTCT177300.038.9993949
CACGGCT179700.038.68367431
TGCCGTC180200.038.4678548
Produced by FastQC (version 0.11.2)