FastQCFastQC Report
Thu 21 Sep 2017
H2FN2BGX3_n01_lbldam_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FN2BGX3_n01_lbldam_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9653081
Sequences flagged as poor quality0
Sequence length75
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCAGATCGGAAGAGCACAC165984717.194997120608434No Hit
GGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACAC158386916.407911629457995No Hit
GGTCGCGGCCGAGGATCCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACA3283233.4012249560528915No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGATCCTCGGCCGCGACCAGATCGGAAGAGCACA3282243.4001993767585703No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1341101.389297365266074Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1323831.3714067042429252Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTATCCTCGGCCGCGACCAGATCGGAAGAGCACACGT519770.5384498482919599No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGCTCCTCGGCCGCGACCAGATCGGAAGAGCACAC497720.5156074003730001No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGTCCTCGGCCGCGACCAGATCGGAAGAGCACACG480380.4976442236421718No Hit
GGTCGCGGCCGAGGATAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGT453370.4696635198647976No Hit
GGTCGCGGCCGAGGACTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACG415480.4304118032367075No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGA399760.4141268471693131No Hit
TCCTCGGCCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATGCCGTCTTCTG313390.32465282327994555RNA PCR Primer, Index 42 (100% over 39bp)
GGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATGCCGTCTTCTG303440.3143452334026825RNA PCR Primer, Index 42 (100% over 39bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGGCCGCGACCAGATCGGAAGAGCACAC290860.30131312479404243No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCG278370.28837425066670425No Hit
GGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACG263070.2725243888453852No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGATCCTCGGCCGCGACCAGATCGGAAGAGCACC253050.262144283260443No Hit
GGTCGCGGCCGAGGAGCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACAC252520.261595235759443No Hit
GGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTA242520.2512358489481234No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCAGATCGGAAGAGCACACG237950.24650160917535033No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTCCTCGGCCGCGACCAGATCGGAAGAGCACACGTCT225770.23388387603916305Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGCCCAGATCGGAAGAGCACAC202620.20990189557095812No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTTCCTCGGCCGCGACCAGATCGGAAGAGCACACGTC198580.205716703299185No Hit
GGTCGCGGCCGAGGAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCT188010.19476683143962017Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTCGCGGCCGAGGATCAGAACGCAAACGCGGGAATAGCGGTTGGAGATTGTAGACGTACACCGTGGAAATTGAG179300.1857438055269608No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTAATACGACTCACTATAGGGCAGCGTGGTCGC168630.17469033979928275No Hit
GGTCGCGGCCGAGGATCTTACATTCATTTAATATTAGAAAAAGCCACAAATTCATAACACAACAAGCCAAGAAAA166690.17268061875788673No Hit
GGTCGCGGCCGAGGAAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTC166530.17251486856890563No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGTCCTCGGCCGCGACCACGCTGCCCTATAGTGAG164670.17058802262200015No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCG153150.15865400901535998No Hit
GGTCGCGGCCGAGGATCTCTGATACGTATCTCCATAATAATGTGTGAGTAGTTCCCAGATAAGGGAATTAGGGTT150950.15637494391686965No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAATACGACTCACTATAGGGCAGCGTGGTCGCGGC148760.15410623820519065No Hit
GGTCGCGGCCGAGGACCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACAC145360.15058404668934197No Hit
GGTCGCGGCCGAGGATCTGGATTTTAGTACTGGATTTTGGTTTTAGGAATTAGAAATTTTATTGATAGAAGTATT143980.14915445130937988No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATCCTCGGCCGCGACCAGATCGGAAGAGCACACGTCTG126140.13067330523798568Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATAATACGACTCACTATAGGGCAGCGTGGTCGCG124430.12890185009325003No Hit
GGTCGCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTC123050.1274722547132879Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGTTCCTCGGCCGCGACCAGATCGGAAGAGCACAC119700.12400186013149583No Hit
GGTCGCGGCCGAGGATCCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACC116780.1209769191825905No Hit
GGTCGCGGCCGAGGATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTG115720.11987882418059063Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTC113400.11747544644036448No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGT111810.11582830393736467No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAATCCTCGGCCGCGACCAGATCGGAAGAGCACA109030.1129483944038178No Hit
ACCCTTATTCCACTGCACCGTGCTGACCCATTCCTATTCAATCTAGAAATCGATAATATCGTAAGAGAAGAAAAT107210.11106298600415762No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAATACGACTCACTATAGGGCAGCGTGGTCGCGG105270.10905326496276162No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG99700.1032830865088566RNA PCR Primer, Index 42 (100% over 43bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTC98670.10221606966729069Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGTCGCGGCCGAGGAACTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACAC97240.10073467735327198No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG96880.10036173942806446No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCACTG123300.068.137829
CTGCACC124550.067.4280313
TGCACCG124650.067.29092414
CCTTATT124700.067.2376863
TTCCACT124900.067.0685358
ATTCCAC125000.067.0428167
TATTCCA125350.066.94376
ACTGCAC125700.066.8656812
TTATTCC125700.066.702445
ACCCTTA117400.066.5997851
GTCCGTT4150.066.4903567
ACTTGGG12800.066.3192953
GTACGCA13100.066.0782318
CTTATGC38900.066.0628260
CTTATTC126950.066.018834
ACCCATT18800.065.86240426
TGTACCT13050.065.80968526
TTATGCG28200.065.8077361
CTGACCC18900.065.6906323
GCAATGT13200.065.57899522
Produced by FastQC (version 0.11.2)