FastQCFastQC Report
Thu 21 Sep 2017
H2FN2BGX3_n01_lbldam_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FN2BGX3_n01_lbldam_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10558876
Sequences flagged as poor quality0
Sequence length75
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCAGATCGGAAGAGCACAC147586813.977510485017532No Hit
GGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACAC140819813.336627875921641No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3885123.679482550983646Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3741303.5432748713025894Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGTCGCGGCCGAGGATCCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACA3500773.3154760033170194No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGATCCTCGGCCGCGACCAGATCGGAAGAGCACA3484653.30020922681543No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGTCCTCGGCCGCGACCAGATCGGAAGAGCACACG766640.7260621300979385No Hit
GGTCGCGGCCGAGGACTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACG666400.6311277829193184No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTATCCTCGGCCGCGACCAGATCGGAAGAGCACACGT573900.5435237614306674No Hit
GGTCGCGGCCGAGGATAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGT532310.5041350992283649No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGCTCCTCGGCCGCGACCAGATCGGAAGAGCACAC501320.47478538435341033No Hit
ACCCTTATTCCACTGCACCGTGCTGACCCATTCCTATTCAATCTAGAAATCGATAATATCGTAAGAGAAGAAAAT457310.4331048115348641No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGGCCGCGACCAGATCGGAAGAGCACAC429050.4063405991319531No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTAATACGACTCACTATAGGGCAGCGTGGTCGC423760.4013305961733048No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGTCCTCGGCCGCGACCACGCTGCCCTATAGTGAG380970.3608054493679062No Hit
GGTCGCGGCCGAGGATCGTACGCAATGTACCTGACCTAGATTGCGAGGCACTACTTGGGCCCTAGCTTCTCGATG374750.35491467084185857No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGA372930.35319100252716296No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCC359190.3401782538217136Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCG317620.3008085330294626No Hit
GGTCGCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTC303800.28772001868380687Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGATCCTCGGCCGCGACCAGATCGGAAGAGCACC293560.2780220167373876No Hit
ACCCTTATTCCACTGCACCGTCGTCGAGCTCTCCGTCGAGTAACTGCAGTCTAACTCTATCTGCAAGTCCGTTCA273920.2594215520667162No Hit
GGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACG272790.2583513623988008No Hit
GGTCGCGGCCGAGGAGCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACAC258780.24508290465765484No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTC242210.2293899464299041Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGTCGCGGCCGAGGATCGAGTATCTCTTCAGTGAACCGGAATTAACAGACCTGAAACCTTGAATTGATGGTCGAG237950.22535542608891324No Hit
ACCCTTATTCCACTGCACCGGAGCAGCTTGAGAATATGGATGCTAGAGCAAAAGGAGTACTGAAAGTAATCTGTA231800.21953094249804617No Hit
GGTCGCGGCCGAGGACCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACAC228530.21643402195460956No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCAGATCGGAAGAGCACACG217410.20590259796591986No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCC216010.20457669926230784Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATAATACGACTCACTATAGGGCAGCGTGGTCGCG197970.18749154739576449No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTCCTCGGCCGCGACCAGATCGGAAGAGCACACGTCT194730.18442303896740525Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGCCCAGATCGGAAGAGCACAC192410.18222583540141962No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAATACGACTCACTATAGGGCAGCGTGGTCGCGGC187480.1775567778237002No Hit
GGTCGCGGCCGAGGATCACCGGAAAACCGCCGGTGATTTAGCCGGACCGAGCGCTAGTGTTAAAGCTGGAGCTTT185080.17528380861750814No Hit
TCCTCGGCCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAATCTCGTATGCCGTCTTCTG173820.16461979475845726RNA PCR Primer, Index 36 (100% over 39bp)
GGTCGCGGCCGAGGAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCT170760.1617217590205624Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAATCTCGTATGCCGTCTTCTG168150.1592499050088286RNA PCR Primer, Index 36 (100% over 39bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCG167050.15820812745599058No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGT155330.14710846116575288No Hit
ACCCTTATTCCACTGCACCGTCATCAACAGCAGATATTTTTATATTCCTTTTACAGAGCCGAAACTCTCCCCCAA152290.14422936683790966No Hit
GGTCGCGGCCGAGGATCCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACC145210.13752410767964318No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTTCCTCGGCCGCGACCAGATCGGAAGAGCACACGTC144530.13688009973788878No Hit
GGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTA142720.13516590212821894No Hit
ACCCTTATTCCACTGCACCGTCAAGTTGCTGCTTAGTGTTTAAAGAACCAAGAAGACCGAGTAGCAACTCCAACC130870.12394311667264585No Hit
GGTCGCGGCCGAGGAAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTC126290.1196055337708294No Hit
GGTCGCGGCCGAGGATCGTTTCCTCAAGAGCACGCAAGAGGCGGTTGGAAATGTGAAATAGTCATGACTCAATGA123460.11692532424852797No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAATACGACTCACTATAGGGCAGCGTGGTCGCGG119540.11321280787841433No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTC113900.10787133024386308No Hit
GGTCGCGGCCGAGGATCAACGAACCATATTCATAGATGACAACGGCATGTGACAGTTTTTTTATATTTTCATGAG112600.10664013859050907No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCTTA491100.068.458941
CCTTATT546200.068.233453
CCCTTAT547350.068.128232
TATTCCA546150.068.1203466
TGCACCG543900.068.087114
CTTATTC547550.068.078464
CTGCACC546050.068.0340713
ACTGCAC546450.067.99625412
ATTCCAC547250.067.983157
TTATTCC548200.067.909655
TCCACTG547300.067.897279
GTGCTGA58500.067.6150520
CACTGCA550250.067.4765311
TTCCACT551950.067.3786248
CCGTGCT60400.067.1444818
GCACCGG159450.067.028215
TCGTACG49600.066.9555616
CGCAATG49800.066.89345621
ATCGTAC49800.066.7587515
GTACGCA49700.066.7511218
Produced by FastQC (version 0.11.2)