FastQCFastQC Report
Thu 21 Sep 2017
H2FN2BGX3_n01_lbldam_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FN2BGX3_n01_lbldam_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2731193
Sequences flagged as poor quality0
Sequence length75
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2525989.24863237420424Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2463299.019098979823104Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCAGATCGGAAGAGCACAC1107104.053539973191202No Hit
GGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACAC1105664.048267551945249No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTATCCTCGGCCGCGACCAGATCGGAAGAGCACACGT759882.7822274002606187No Hit
GGTCGCGGCCGAGGATAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGT756182.768680206781432No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGTCCTCGGCCGCGACCAGATCGGAAGAGCACACG623712.2836540661901226No Hit
GGTCGCGGCCGAGGACTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACG605302.216247625122062No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTCCTCGGCCGCGACCAGATCGGAAGAGCACACGTCT350951.284969608519061Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTCGCGGCCGAGGAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCT343281.2568866425770717Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTTCCTCGGCCGCGACCAGATCGGAAGAGCACACGTC291141.0659810566298318No Hit
GGTCGCGGCCGAGGAAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTC290241.0626857933511107No Hit
GGTCGCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTC188970.6918954464221313Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGTCGCGGCCGAGGATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTG181770.6655333401923629Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATCCTCGGCCGCGACCAGATCGGAAGAGCACACGTCTG180670.661505796185037Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTAATACGACTCACTATAGGGCAGCGTGGTCGC163680.5992985482900696No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAATACGACTCACTATAGGGCAGCGTGGTCGCGGC145200.5316358089669972No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTC141100.5166240540306013Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGTCCTCGGCCGCGACCACGCTGCCCTATAGTGAG132050.4834883510612395No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCG120530.44130898109361No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATAATACGACTCACTATAGGGCAGCGTGGTCGCG117370.4297389455816561No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAATACGACTCACTATAGGGCAGCGTGGTCGCGG106450.3897564177998406No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTC97300.3562545744661765No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGT97190.3558518200654439No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA86750.3176267660322797TruSeq Adapter, Index 27 (97% over 44bp)
GGTCGCGGCCGAGGATCCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACA80880.2961343266477323No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGATCCTCGGCCGCGACCAGATCGGAAGAGCACA77630.28423476480790627No Hit
GGTCGCGGCCGAGGACTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTAATACGACTCACTATA54200.19844807745186813No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC54150.19826500726971694Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC53770.19687367388536806Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC53670.1965075335210657Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC53650.19643430544820525Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTCGCGGCCGAGGATCAGAACGCAAACGCGGGAATAGCGGTTGGAGATTGTAGACGTACACCGTGGAAATTGAG46310.1695596027084135No Hit
GGTCGCGGCCGAGGATCTTACATTCATTTAATATTAGAAAAAGCCACAAATTCATAACACAACAAGCCAAGAAAA43540.1594175146172387No Hit
GGTCGCGGCCGAGGAGTCTTATTTCTCAATGCATTCATAATTCAGACAGAGAAATACGACTCACTATAGGGCAGC38850.14224553153145894No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCC38000.1391333384348891Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGT37270.136460513775482No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATACGACTCACTATAGGGCAGCGTGGTCGCGGCC36400.13327509260605164No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTTCTCTGTCTGAATTATGAATGCATTGAGAAATAAG33780.12368221506133034No Hit
GGTCGCGGCCGAGGATAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATAATACGACTCACTATAGG32760.11994758334544647No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCC29300.10727912674058553TruSeq Adapter, Index 27 (100% over 38bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29190.10687637233985296No Hit
GGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTC28310.10365433713399237TruSeq Adapter, Index 27 (97% over 44bp)
CGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCC27410.1003590738552713TruSeq Adapter, Index 27 (100% over 38bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAG150.00236005168.9693422
GTGCCTA150.002360222468.9680718
ATCTAAT164350.064.8650915
GATCTAA165450.064.80904414
GGATCTA167700.064.55649613
TCTAATA165150.064.4602616
TGCATTC4500.062.896330
GTCTAAC550.062.76719349
AGGATCT208300.062.33865412
GTATTCC88000.061.07808336
CCAGTCC9450.060.9500869
CGTATTC93800.060.9459635
GATCCTA13600.060.86309414
TCCACTG3350.060.7408689
CGTATCC36550.060.54218335
TAGATCC154300.060.20788240
ATTAGAT157500.060.0637538
TTAGATC156400.059.99851639
TCGTATC38250.059.83476634
ATCCTAA13150.059.7985715
Produced by FastQC (version 0.11.2)