FastQCFastQC Report
Thu 21 Sep 2017
H2FN2BGX3_n01_lbldam_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FN2BGX3_n01_lbldam_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5706659
Sequences flagged as poor quality0
Sequence length75
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4893968.575876007310058Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4784548.384135095508597Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCAGATCGGAAGAGCACAC1673352.9322761356513505No Hit
GGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACAC1661512.9115284442262976No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTATCCTCGGCCGCGACCAGATCGGAAGAGCACACGT1610112.8214582297628086No Hit
GGTCGCGGCCGAGGATAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGT1600012.803759607854613No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGTCCTCGGCCGCGACCAGATCGGAAGAGCACACG1238992.171130253270784No Hit
GGTCGCGGCCGAGGACTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACG1207522.115984151146932No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTCCTCGGCCGCGACCAGATCGGAAGAGCACACGTCT691171.211164010325481Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTCGCGGCCGAGGAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCT675471.1836522911216527Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTCGCGGCCGAGGAAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTC603161.0569406722917911No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTTCCTCGGCCGCGACCAGATCGGAAGAGCACACGTC602871.0564324940389815No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTAATACGACTCACTATAGGGCAGCGTGGTCGC497460.8717184608367172No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAATACGACTCACTATAGGGCAGCGTGGTCGCGGC456090.799224204565228No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGTCCTCGGCCGCGACCACGCTGCCCTATAGTGAG388130.6801352595275099No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATAATACGACTCACTATAGGGCAGCGTGGTCGCG381450.6684296363248619No Hit
GGTCGCGGCCGAGGATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTG376090.6590371003418989Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCG365740.6409003937330057No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATCCTCGGCCGCGACCAGATCGGAAGAGCACACGTCTG363220.6364844999499708Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAATACGACTCACTATAGGGCAGCGTGGTCGCGG341840.5990194963462859No Hit
GGTCGCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTC333290.5840369995824176Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGT315850.5534762108617318No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTC314560.5512156938061308No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTC252820.44302629612177635Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGTCGCGGCCGAGGACTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTAATACGACTCACTATA138550.24278654112677836No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATACGACTCACTATAGGGCAGCGTGGTCGCGGCC118510.20766967151883442No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGT114100.19994185739852338No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCC103100.1806661305678156Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC101730.1782654264079911Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC100210.17560187142774783Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC99670.17465560847424036Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA94550.16568363380394727TruSeq Adapter, Index 25 (97% over 44bp)
GGTCGCGGCCGAGGATAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATAATACGACTCACTATAGG93130.1631953127039832No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC87180.15276889682737307Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGTCCTCGGCCGCGCCCACGCTGCCCTATAGTGAG75430.1321789158945716No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTCCTCGGCCGCGCCCACGCTGCCCTATAGTGAGTCG75150.13168826102978995No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGCTAATACGACTCACTATAGGGCAGCGTGGTCGCG74970.13137284004528746No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG62020.10868005254913603No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGCCTCGGCCGCGACCACGCTGCCCTATAGTGAGT59490.10424663537807323No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTCC24950.064.5550469
ATCTAAT283000.061.82526415
GATCTAA285000.061.70659314
TCTAATA284350.061.4816116
GGATCTA292300.060.75494813
TATTATC364200.060.36012337
TTATCCT356850.060.34609239
ATTATCC359650.060.207838
TAGATCC258950.060.16798440
GTATTAT376400.060.0666436
TATTTCC185550.059.89819337
GTATTTC189200.059.89511536
AGATCCT258500.059.87381441
ATTAGAT266100.059.72733338
TTAGATC264550.059.6676139
ATTTCCT184000.059.66699238
CGTATTT199650.059.6657235
CGTATCC88200.059.2916235
GATCTAC5950.059.13635357
GTATTCC216450.059.11622236
Produced by FastQC (version 0.11.2)