FastQCFastQC Report
Thu 21 Sep 2017
H2FN2BGX3_n01_lblchip_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FN2BGX3_n01_lblchip_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22269344
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA809540.36352215853327335TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG130900.050.02634448
GCCGTCT134200.048.73630551
GTATGCC135400.048.38976347
CTCGTAT141850.045.701444
AGTCACG150300.043.82568428
TATCTCG147850.043.64938741
TCTCGTA151500.042.78682743
ATCTCGT155150.041.6886342
GAGTGGA165300.039.8204334
CGTATGC176200.037.47874546
GCTTGAA177400.037.19537761
ATGCCGT178150.036.85444649
TCGTATG187400.035.16497845
ACGAGTG192100.034.44419532
CGAGTGG192650.034.23851433
ATATCTC190800.034.0750140
CCGTCTT200450.033.14087752
ACGTCTG203450.032.531215
CGTCTGA204650.032.355716
CAGTCAC206200.032.08767727