FastQCFastQC Report
Thu 21 Sep 2017
H2FN2BGX3_n01_lblchip_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FN2BGX3_n01_lblchip_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11791744
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA450430.38198760081629995TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG74900.050.6988448
GCCGTCT75950.049.7176251
GTATGCC77200.049.18859547
CCGTACG80100.047.2313333
CTCGTAT78300.046.60076544
CGTACGT81350.046.46394734
TACGTAA84650.044.4904236
GTACGTA85050.044.40369435
CACCGTA85650.044.08926831
TCTCGTA82850.043.99611743
ATCTCGT85300.042.72900442
AATCTCG85150.042.4380341
AGTCACC91300.041.4245128
GTCACCG92450.041.06497629
TCACCGT92800.040.98795730
GCTTGAA94900.039.72579261
CGTATGC101200.037.65976346
ATGCCGT103800.036.6160649
TCGTATG104800.036.03639645
ACCGTAC111000.034.45617732
Produced by FastQC (version 0.11.2)