FastQCFastQC Report
Thu 21 Sep 2017
H2FN2BGX3_n01_lblchip_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FN2BGX3_n01_lblchip_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23059355
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA2957011.2823472295734204TruSeq Adapter, Index 19 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA396960.17214705268208932TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG427100.057.18337248
GCCGTCT432100.056.38467851
GTATGCC433900.056.28793347
CTCGTAT427200.055.14032444
TCTCGTA429850.054.00929343
GTCACGT458300.053.43506629
AGTCACG459850.053.33656728
ACGATCT425750.053.27129439
TCACGTG460000.053.2420930
CGTATGC461050.053.13017746
CACGTGA462050.053.08241331
CGATCTC429700.052.7801140
ATCTCGT431550.052.6823742
ATGCCGT468350.052.15340449
TCGTATG469350.051.97698645
CGTGAAA476200.051.46384433
GAAACGA478350.050.2368636
GCTTGAA490350.049.9082561
AAACGAT477800.049.3743237
GTGAAAC499750.049.04601734