Basic Statistics
Measure | Value |
---|---|
Filename | H2FN2BGX3_n01_lblchip_02.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 23059355 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 42 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA | 295701 | 1.2823472295734204 | TruSeq Adapter, Index 19 (97% over 40bp) |
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA | 39696 | 0.17214705268208932 | TruSeq Adapter, Index 19 (97% over 40bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 42710 | 0.0 | 57.183372 | 48 |
GCCGTCT | 43210 | 0.0 | 56.384678 | 51 |
GTATGCC | 43390 | 0.0 | 56.287933 | 47 |
CTCGTAT | 42720 | 0.0 | 55.140324 | 44 |
TCTCGTA | 42985 | 0.0 | 54.009293 | 43 |
GTCACGT | 45830 | 0.0 | 53.435066 | 29 |
AGTCACG | 45985 | 0.0 | 53.336567 | 28 |
ACGATCT | 42575 | 0.0 | 53.271294 | 39 |
TCACGTG | 46000 | 0.0 | 53.24209 | 30 |
CGTATGC | 46105 | 0.0 | 53.130177 | 46 |
CACGTGA | 46205 | 0.0 | 53.082413 | 31 |
CGATCTC | 42970 | 0.0 | 52.78011 | 40 |
ATCTCGT | 43155 | 0.0 | 52.68237 | 42 |
ATGCCGT | 46835 | 0.0 | 52.153404 | 49 |
TCGTATG | 46935 | 0.0 | 51.976986 | 45 |
CGTGAAA | 47620 | 0.0 | 51.463844 | 33 |
GAAACGA | 47835 | 0.0 | 50.23686 | 36 |
GCTTGAA | 49035 | 0.0 | 49.90825 | 61 |
AAACGAT | 47780 | 0.0 | 49.37432 | 37 |
GTGAAAC | 49975 | 0.0 | 49.046017 | 34 |