FastQCFastQC Report
Thu 21 Sep 2017
H2FN2BGX3_n01_lblchip_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FN2BGX3_n01_lblchip_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16797846
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA2321171.381825979354734TruSeq Adapter, Index 18 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA237050.14111928398438703TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG334350.058.88350348
GTATGCC334950.058.80946447
CGCACAT326150.058.59833537
GCCGTCT336500.058.1287751
CCGCACA334650.057.96983736
GTCCGCA339350.057.7467734
CTCGTAT333450.057.6335844
TCCGCAC341950.057.35867735
TCTCGTA336800.056.36065743
AGTCACG350800.056.2844428
GTCACGT351350.056.13711529
CACGTCC353550.055.58098631
CGTATGC356200.055.43639446
ATCTCGT341850.055.0197342
TCGTATG361850.054.25580245
ATGCCGT366400.053.65614349
ACGTCCG372700.052.7731732
CGTCCGC376000.052.37473333
TCACGTC376900.052.28209730
GCTTGAA375850.052.1530361
Produced by FastQC (version 0.11.2)