FastQCFastQC Report
Fri 8 Sep 2017
H2FMNBGX3_n02_rice2016dsproject_plate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FMNBGX3_n02_rice2016dsproject_plate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3772104
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATATGTTGAGCGAGATAACAAAACACAGGCGTGCCCGAGGGTTAATT560081.484794692829254No Hit
CGGTTTAGGCTGCTCCCATTTCGCTCGCCGCTACTACGGGAATCGCTTTT398091.0553526625989103No Hit
AGTACTACCTGAGGATATCGTCCCCGATCATCGATGTGAACGCGTGGAGT264220.7004578876934464No Hit
CCCACAAGGAGGACGACCTCATCTAATCAATCAAGCAAGCTCCATATTTA175160.4643562319596703No Hit
CGTAGAAGCTGCTGCTGCTGCTGCCTTTGTAACTCCACCTCGCTCCAAAA174790.46337534702118505No Hit
CCTACAAGCCCCCGGGCTGCGAGGAGTCTGGTGGCAACTAAGCCGTCATC131890.3496457149643806No Hit
GGTACCAGCTATGGCGTCGTCATAGTTGAAGCCGAGAAGAGCCACTTCGA113240.300203811983975No Hit
CATACGTGGTTCATCATGTATTTCATCACATGAATCTTTTCTAGAGCGTT111780.29633329303751965No Hit
CATCGTAGGCGGCCTGCATAGGAGGAGGAGGGCAAGGAGCACAGCAAGCA101080.2679671610326757No Hit
CATCATAGGATTCCGGTCCTATTGTGTTGGCCTTCGGGATCGGAGTAATG90180.23907082095297477No Hit
CGTCATATATAGCCTCGTTTAATTGTTCATCTCTGATTCGATGATGTCTC80560.21356781255235804No Hit
CATATAAGTAGTAAGCCCACCCCAAGATGAGTGCTCTCTCCTCCGACTTC77030.20420964003113382No Hit
GGTCCTTGCTGATTCACACGGGATTCCACGTGCCCCATGCTACTCGGGTC72450.19206787511691087No Hit
CTTACGAGGATTCCCCTAGTAACGGCGAGCGAACCGGGAGATGCCCAGCT71640.18992053241374046No Hit
CGTACCATGTGTACACTTGTAGTAGCCACGCACCGACCCTGCAGTTGCAG68760.18228553613580115No Hit
CGTATACGTTGAGCGAGATAACAAAACACAGGCGTGCCCGAGGGTTAATT67020.17767272588454613No Hit
CGTCATAGTTGAAGCCGAGAAGAGCCACTTCGAGGAGGTCGCCGCCGGCG66860.1772485594246606No Hit
TGTAATAATCTCTTCCCTCCCTCTCCGACCGGTTTTATTGTAAGAGTACT63470.1682615325558362No Hit
CATAGGAGGAGGAGGGCAAGGAGCACAGCAAGCAGGTAACCTGCCTGTGC62170.1648151800692664No Hit
GGTACGCGCAGCTCGAGTAGGGAATGAACAAATGTTTTGTGCGTGGCCGG56890.15081768689304428No Hit
GGAAATAGCTAATGTAGAATTTATCTGATATAGAACACTCATATCGATAA56050.14859081297864535No Hit
CGTCCGAGGAGGTGCGGCAGCTGGAGGAGCAGGCCGCCGCCGTGCTGAAG49280.13064326964473938No Hit
GGTATGAGCCTCGTGGAGAAGCAATGGCTAGTCCACGGGGCTCATACTTC41830.11089301885631997No Hit
CCTACAAGCCCCCAGGTTGCGAGGAGTCTGGTGGCAACTAAGCTAAGATC40350.10696947910237894No Hit
CTTGCAAGGTGGTCCTTGCTGATTCACACGGGATTCCACGTGCCCCATGC39860.10567046931897954No Hit
CATATTACCTGCATAATTAATAAGTATAGTTTATCACCTGGCTATGCTAT39830.10559093810775101No Hit
GGTTCAAGGGCAGGACCGAGGGCTACTTCGACAAGTGAATGAATCCACAG38120.10105765906772454No Hit
CGTACGTACCGGATGGTTCGATATATGTGAATGCTGTATTAAGTAATAAC37960.10063349260783902No Hit
CGTATAAGCCACCGGGTTGCGAGGAGTCAGGCGGCAACTAAGCGCTTTCG37750.10007677412923928No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTTA68200.043.0323221
TTTAGGC66300.042.9649664
CCGCTAC61000.042.6647928
TTAGGCT69450.042.570955
GTTTAGG63250.042.4994353
GCCGCTA59550.042.26230627
CATTTCG61100.042.15953417
ATTTCGC61450.042.09839618
ACCTGAG57600.042.0699657
GGGTTAA75950.041.92362242
ATATCGT53500.041.6931115
CGGGAAT57050.041.67732637
TAGGCTG70450.041.66466
TATCGTC54100.041.55655316
GGTTTAG70750.041.474732
CGTGGTT22050.041.2719575
TGAGCGA116650.041.11781710
TGCCCGA88700.041.1014335
GTTGAGC116350.041.0720838
CGCTACT63050.041.0693329
Produced by FastQC (version 0.11.2)