FastQCFastQC Report
Fri 8 Sep 2017
H2FMNBGX3_n01_rice2016dsproject_plate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FMNBGX3_n01_rice2016dsproject_plate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3772104
Sequences flagged as poor quality0
Sequence length20
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCC66930.17743413225086052No Hit
GGGGGGGGGGGGGGGGGGGG46240.12258410690691454No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCACC31300.014.000732
GGCAGTA905.456968E-1213.99924514
CCCACCA300.005940675713.99924512
AAGCCCA401.7562066E-413.99924514
CCTCCTC453.0382434E-513.99924512
CCCCACC350.00101911813.99924414
GGTCACT505.2746254E-613.9990613
TCGCCCC300.005941197713.99905913
TCCCTCT401.7564042E-413.99905911
GGCCCAT350.001019220113.99905913
CCCAACT350.001019220113.99905913
TCCCACC350.001019220113.99905911
ACACCTT75450.013.9543392
CTCCAGT35950.013.9423115
AACTACC68500.013.9394116
AGTCTCC62500.013.9238011
CCTCTCT42950.013.9192344
TCAGAGC68750.013.9105631
TACACCT75450.013.9094231
GGTACGT43700.013.9046144
Produced by FastQC (version 0.11.2)