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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.0.dev0

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

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        Report generated on 2017-09-14, 00:09 based on data in: /mnt/gencore/sites/core-fastqc.bio.nyu.edu/html/H2FMMBGX3/merged


        General Statistics

        Showing 19/19 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        H2FMMBGX3_n01_cotylset2_01
        59.6%
        44%
        18.0
        H2FMMBGX3_n01_cotylset2_02
        61.0%
        44%
        21.6
        H2FMMBGX3_n01_cotylset2_03
        62.8%
        44%
        21.1
        H2FMMBGX3_n01_cotylset2_04
        60.2%
        44%
        20.5
        H2FMMBGX3_n01_cotylset2_05
        61.9%
        44%
        25.1
        H2FMMBGX3_n01_cotylset2_06
        48.9%
        44%
        9.5
        H2FMMBGX3_n01_cotylset2_07
        62.8%
        44%
        27.3
        H2FMMBGX3_n01_cotylset2_08
        60.4%
        44%
        19.8
        H2FMMBGX3_n01_cotylset2_09
        61.3%
        44%
        21.8
        H2FMMBGX3_n01_cotylset2_10
        61.2%
        44%
        20.8
        H2FMMBGX3_n01_cotylset2_11
        54.8%
        44%
        16.6
        H2FMMBGX3_n01_cotylset2_12
        65.0%
        44%
        27.3
        H2FMMBGX3_n01_cotylset2_13
        65.5%
        44%
        26.4
        H2FMMBGX3_n01_cotylset2_14
        73.5%
        44%
        52.1
        H2FMMBGX3_n01_cotylset2_15
        76.3%
        44%
        43.2
        H2FMMBGX3_n01_cotylset2_16
        67.2%
        44%
        28.8
        H2FMMBGX3_n01_cotylset2_17
        58.2%
        44%
        18.4
        H2FMMBGX3_n01_cotylset2_18
        66.3%
        44%
        31.9
        H2FMMBGX3_n01_tga1ox_60n_r3
        52.8%
        45%
        11.4

        Run Statistics

        Run Statistics

        Showing 1/1 rows and 3/3 columns.
        Number of LanesTotal # of Single-End ReadsTotal # PF ReadsUndetermined
        4.0
        506510614
        474576355
        2.7%

        Demultiplexing Report

        Demultiplexing Report
        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool.
        Perfect: The percentage of reads in this library which have a barcode perfectly matching the barcode specified in TuboWeb. The remainder of reads have mismatches upto the max number of mismatches specified in TuboWeb (Allowed barcode mismatch option in your Library Pool).

        Showing 19/19 rows and 3/3 columns.
        LibraryTotal Read CountPortion (%)Perfect (%)
        cotylset2_06
        9466052
        2.0
        99.1
        cotylset2_10
        20789151
        4.5
        99.0
        cotylset2_04
        20497127
        4.4
        98.5
        cotylset2_07
        27328766
        5.9
        99.1
        cotylset2_12
        27304771
        5.9
        99.0
        cotylset2_05
        25095155
        5.4
        99.2
        cotylset2_09
        21782241
        4.7
        99.2
        cotylset2_03
        21058111
        4.6
        99.1
        cotylset2_01
        18002180
        3.9
        99.0
        cotylset2_08
        19797686
        4.3
        99.2
        cotylset2_11
        16594216
        3.6
        99.1
        cotylset2_18
        31943657
        6.9
        99.2
        cotylset2_13
        26434701
        5.7
        98.9
        cotylset2_16
        28775528
        6.2
        99.1
        cotylset2_15
        43243122
        9.4
        99.2
        cotylset2_17
        18415681
        4.0
        98.9
        cotylset2_02
        21636317
        4.7
        99.1
        cotylset2_14
        52145725
        11.3
        99.1
        tga1ox_60n_r3
        11445764
        2.5
        99.1

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Quality Histograms

        The mean quality value across each base position in the read. See the FastQC help.

        loading..

        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.

        loading..

        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.

        Click a heatmap row to see a line plot for that dataset.

        rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.

        loading..

        Per Base N Content

        The percentage of base calls at each position for which an N was called. See the FastQC help.

        loading..

        Sequence Length Distribution

        All samples have sequences of a single length (75bp).


        Sequence Duplication Levels

        The relative level of duplication found for every sequence. See the FastQC help.

        loading..

        Overrepresented sequences

        The total amount of overrepresented sequences found in each library. See the FastQC help for further information.

        loading..

        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.

        No samples found with any adapter contamination > 0.1%