FastQCFastQC Report
Thu 14 Sep 2017
H2FMMBGX3_n01_tga1ox_60n_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FMMBGX3_n01_tga1ox_60n_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11445764
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG189830.16585175091850574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG37150.037.703538
TGTGTGC48750.029.2984267
TGTGCGA48050.029.0069949
GTGCGAG50000.027.73772410
CTGACAT54300.027.7019861
TGCGAGT52200.026.63479411
ACATGTG57050.026.063954
ATGTGTG58800.025.1708876
GCGAGTC56100.024.84478212
CGGGCGC14200.023.81045515
GACATGT62050.023.6301083
GCCAGTA30950.022.62871
ACCCGTA61600.022.34627232
TGCCCGC40200.021.7993416
CCCTACT40750.021.6735947
ACGGGTG64350.021.60688420
TGACATG65950.021.5526662
CCCGTAA65500.021.27908533
CGGGTGA65000.021.23139221
CGAGTCA66100.021.08629813
Produced by FastQC (version 0.11.2)