FastQCFastQC Report
Thu 14 Sep 2017
H2FMMBGX3_n01_cotylset2_18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FMMBGX3_n01_cotylset2_18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31943657
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG136650.015.98135
CACCTTA211450.015.5979287
ACCTTAG215650.015.3260198
CCTTAGA235150.014.216489
CGGGAAT121650.013.6417921
TAGAGTA250200.013.48543212
CTAGTTG89950.012.8486348
GGACACC79950.012.72970129
CGGGGCC57150.012.55630329
GGGGCCG56700.012.47341830
CTTAGAG278650.012.09619610
TTAGAGT277700.011.98846111
TACTCTA100900.011.5911524
GGGGTGT63500.011.51792146
GGGCCGT62350.011.39836931
TCTAGTT126100.011.2993037
AGAGTAC300900.011.21328213
TACCCTA288500.011.18092540
CCACCTT302800.011.1429736
GTCGGGC66950.011.13105215
Produced by FastQC (version 0.11.2)