FastQCFastQC Report
Thu 14 Sep 2017
H2FMMBGX3_n01_cotylset2_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FMMBGX3_n01_cotylset2_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18415681
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG80200.019.185755
CACCTTA122800.016.7441757
ACCTTAG123500.016.5373958
GCTGACT53450.015.2338091
CCTTAGA137550.014.9234339
TAGAGTA146850.014.02535712
CGGGAAT76350.013.9634791
GACTCTA69550.012.8971724
TTAGAGT163800.012.53185711
CTTAGAG166400.012.48117610
CTAGCAG63950.012.3000438
GGACACC45450.012.29690629
ACGGAAC173300.011.82516922
CTAGTTG52250.011.8189558
AGAGTAC175550.011.7914913
CCACCTT174900.011.7761356
TACCCTA167100.011.768187540
CAAACGG177900.011.53942119
CCCTACA172900.011.3734242
TCCACCT182800.011.267215
Produced by FastQC (version 0.11.2)