FastQCFastQC Report
Thu 14 Sep 2017
H2FMMBGX3_n01_cotylset2_16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FMMBGX3_n01_cotylset2_16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28775528
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC615470.21388660531268094No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATC311260.1081683018987523No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC307200.10675738078550635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG146550.024.7655055
CTAGTTG102600.022.528918
GGACACC104100.020.7461329
CACCTTA326550.019.7670617
TACTCTA117050.019.512094
ACCTTAG333650.019.3255068
TCTAGTT127650.019.3242767
CTCTAGT133400.018.206826
GACACCT118400.018.18219630
CCTTAGA359750.017.9713829
ACCTCTA125900.017.9226617
TTTTTCC375550.017.1794781
GTTACCT134650.017.06440514
CTAATGC131250.017.00761221
TTTCCAC392000.016.8011053
TAGAGTA390850.016.53259812
TTTTCCA419750.016.5122872
TCCACCT398700.016.293765
CCACCTT403200.016.0947976
TTAGAGT408100.015.90983811
Produced by FastQC (version 0.11.2)