FastQCFastQC Report
Thu 14 Sep 2017
H2FMMBGX3_n01_cotylset2_15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FMMBGX3_n01_cotylset2_15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43243122
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC1120080.25901922622515555No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC535440.12382084716270024No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATC531400.12288659454328946No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTA461110.10663198646943207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG227400.025.1088435
CTAGTTG170050.023.5338768
GGACACC170300.021.83824729
CACCTTA539600.021.4889037
ACCTTAG547600.021.1242988
TACTCTA190950.021.1209774
TCTAGTT210750.020.1842757
ACCTCTA198850.019.744917
CCTTAGA589200.019.6913859
CTCTAGT212600.019.6679256
TTTTTCC602400.019.324261
GACACCT196950.019.0759230
GTTACCT209600.018.87966714
TTTCCAC636250.018.5283553
CTAATGC211050.018.30907221
TCCACCT638750.018.2397695
TAGAGTA643900.018.16864412
CCACCTT650900.017.830396
TTTTCCA688950.017.66192
TTAGAGT666050.017.49707611
Produced by FastQC (version 0.11.2)