FastQCFastQC Report
Thu 14 Sep 2017
H2FMMBGX3_n01_cotylset2_14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FMMBGX3_n01_cotylset2_14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52145725
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC533370.10228451133817779No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG221150.018.2366965
CACCTTA340200.016.7734137
ACCTTAG343650.016.5445948
CCTTAGA380650.014.99986659
TAGAGTA407700.014.04698212
CTAGTTG147950.013.3381718
GCTGACT158300.013.0771741
GGACACC134600.012.78996929
TTAGAGT448850.012.71305611
GACTCTA183500.012.6155174
CTTAGAG455550.012.563930510
CGGGAAT200150.012.5148191
CTAGCAG172800.012.0389568
TACTCTA167650.011.8120954
AGAGTAC493200.011.7447713
CCACCTT492450.011.7346796
TACCCTA466050.011.67379240
ACGGAAC494500.011.59544622
TCTAGTT199050.011.5606937
CCCTACA478750.011.40735342
Produced by FastQC (version 0.11.2)