FastQCFastQC Report
Thu 14 Sep 2017
H2FMMBGX3_n01_cotylset2_13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FMMBGX3_n01_cotylset2_13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26434701
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG125900.018.825585
CACCTTA165700.016.8647177
CTTCCGA157800.016.4854741
ACCTTAG171450.016.2789028
CCGATCT159150.015.9113424
CCTTAGA186800.015.052059
GCTGACT88850.014.9499711
TAGAGTA198650.014.32785212
CTAGTTG73050.013.7903958
CTAGCAG99050.013.6187228
CGGGAAT107500.013.3512511
GACTCTA106700.013.2244084
TTAGAGT224050.012.73431111
CTTAGAG228500.012.42591910
AGAGTAC238150.012.16879813
GGACACC71350.011.99147529
TACTCTA83000.011.9710374
TACCCTA228550.011.89483240
TCTAGCA205600.011.746047
ACGGAAC243700.011.62275622
Produced by FastQC (version 0.11.2)