FastQCFastQC Report
Thu 14 Sep 2017
H2FMMBGX3_n01_cotylset2_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FMMBGX3_n01_cotylset2_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27304771
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC291450.10673958774457402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCTTA191300.016.754147
ACCTTAG196300.016.3095788
ACTCTAG113050.015.8996285
CCTTAGA215000.015.0354699
TAGAGTA230350.014.15340812
CTAGTTG80600.013.055098
CGGGGCC42700.013.0079829
TTAGAGT253400.012.78426111
CTTAGAG259650.012.46322310
GGGGCCG44650.012.2853530
GGACACC76250.012.17094529
TACTCTA85450.011.9912434
TTTCCAC285500.011.8424233
CCACCTT273950.011.8253536
AGAGTAC277950.011.81657713
CGGGAAT95550.011.7350581
TCCACCT277850.011.65936855
TCTAGTT106700.011.5108397
CAAACGG280800.011.43936319
ACGGAAC283500.011.43934122
Produced by FastQC (version 0.11.2)