FastQCFastQC Report
Thu 14 Sep 2017
H2FMMBGX3_n01_cotylset2_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FMMBGX3_n01_cotylset2_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16594216
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG72750.017.7361495
CACCTTA103200.016.4476387
ACCTTAG104550.016.2020658
CCTTAGA115250.014.6679029
TAGAGTA120300.014.10952412
CTAGCAG52750.013.34198958
CGGGAAT66550.013.3237981
GACTCTA60550.012.877014
GCTGACT51800.012.8549541
TTAGAGT134650.012.580214511
CTTAGAG139850.012.13711710
TACCCTA134600.012.02096640
CTAGTTG47000.011.891328
AGAGTAC145350.011.82026413
CCCTACA138900.011.57431442
GGACACC40600.011.55658929
ACCCTAC141750.011.48763241
ACGGAAC149750.011.40418722
CCACCTT148850.011.4034696
TCTAGCA113250.011.3324257
Produced by FastQC (version 0.11.2)