FastQCFastQC Report
Thu 14 Sep 2017
H2FMMBGX3_n01_cotylset2_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FMMBGX3_n01_cotylset2_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20789151
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC289350.13918317299249017No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG89700.019.9230335
CACCTTA166150.018.9369747
ACCTTAG167850.018.6628278
CTTCCGA130800.018.1212021
CCGATCT130200.017.620934
CCTTAGA183300.017.2403459
TAGAGTA198300.015.88411812
CTAGTTG63300.015.7509628
GGACACC58000.014.93006829
TTAGAGT216250.014.5974811
TACTCTA68200.014.4677114
CTTAGAG220650.014.21257910
CCACCTT226150.014.0501586
TTTCCAC234150.013.9826733
TCCACCT230050.013.706995
AGAGTAC231600.013.67477513
TCTAGTT82800.013.6665737
CAAACGG234300.013.28239619
TTCCACC246150.013.1188014
ACGGAAC239750.013.03752222
Produced by FastQC (version 0.11.2)