FastQCFastQC Report
Thu 14 Sep 2017
H2FMMBGX3_n01_cotylset2_09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FMMBGX3_n01_cotylset2_09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21782241
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC454240.2085368534853691No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATC268820.1234124624734434No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC218610.10036157436693496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG107350.022.7856625
CTAGTTG79050.020.7302348
CACCTTA245350.019.6017657
ACCTTAG245750.019.5555538
TACTCTA85150.019.0427954
GGACACC81100.018.46219629
TCTAGTT97900.018.3247957
CCTTAGA268750.018.010339
TTTTTCC281000.017.1770971
CTCTAGT99800.017.042526
TTTCCAC296050.016.5361633
TAGAGTA293600.016.49785612
ACCTCTA97900.016.42292817
GACACCT92100.016.33207330
GTTACCT98950.016.14312714
TTTTCCA321400.016.1121062
TCCACCT301500.015.974075
TTAGAGT303750.015.86695611
CCACCTT307200.015.7338276
CTAATGC97400.015.65686121
Produced by FastQC (version 0.11.2)