FastQCFastQC Report
Thu 14 Sep 2017
H2FMMBGX3_n01_cotylset2_08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FMMBGX3_n01_cotylset2_08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19797686
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC200310.10117849126408006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG81500.016.04355
CACCTTA135350.015.675977
ACCTTAG137800.015.3720698
CCTTAGA148350.014.3719349
TAGAGTA163500.013.16682612
CTAGTTG57200.012.7262258
GGACACC51500.012.32604629
TTAGAGT181650.011.96518611
CTTAGAG180400.011.93334910
TCTAGTT74650.011.831177
TACTCTA64050.011.7423484
TACCCTA185600.011.07851240
CCACCTT193850.011.069866
ACGGAAC196450.011.04656322
AGAGTAC196050.010.98080513
CGGGAAT76550.010.951961
CAAACGG197400.010.9065919
TCCACCT197950.010.85800655
GTCGCCC29200.010.75181162
CCCTACA192000.010.6912642
Produced by FastQC (version 0.11.2)