FastQCFastQC Report
Thu 14 Sep 2017
H2FMMBGX3_n01_cotylset2_07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FMMBGX3_n01_cotylset2_07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27328766
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG121000.018.1908195
CACCTTA165100.015.1080417
GCTGACT86650.014.9711271
ACCTTAG167800.014.8854228
CGGGAAT111450.014.67349151
GACTCTA105400.014.1076144
CCTTAGA181900.013.7315769
CTAGCAG99150.013.29184158
TAGAGTA192950.013.14191612
TCTAGCA204000.011.9903817
TTAGAGT215700.011.77180311
GGGGCCG54250.011.70128130
CGGGGCC54950.011.61500429
CTTAGAG218650.011.455173510
CTAGTTG76200.011.4093288
GGACACC67400.011.36340229
TACCCTA216650.011.16277840
CATGCCG95200.011.1617363
CGGTACC101950.010.96428314
TACTCTA79800.010.9379144
Produced by FastQC (version 0.11.2)