FastQCFastQC Report
Thu 14 Sep 2017
H2FMMBGX3_n01_cotylset2_06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FMMBGX3_n01_cotylset2_06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9466052
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC105440.11138751403436194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG39850.020.0852495
CACCTTA63200.019.8700757
ACCTTAG66000.018.8701868
CCTTAGA71200.017.637399
CTAGTTG26650.016.6995778
TAGAGTA76900.016.5095212
TTAGAGT84050.015.14612811
CTTAGAG85750.014.72515610
AGAGTAC89300.014.44891913
CGGGAAT35950.013.9155511
CCACCTT91350.013.8981436
GGACACC26900.013.46656629
TCCACCT94950.013.4438695
CAAACGG96300.013.22027619
ACCCCGG13800.012.99988441
TTTCCAC97600.012.9374533
GTACAAA101250.012.88049916
GCTGACT29850.012.8295061
CACCCCG13450.012.82516540
ACAAACG100200.012.74001418
Produced by FastQC (version 0.11.2)