FastQCFastQC Report
Thu 14 Sep 2017
H2FMMBGX3_n01_cotylset2_05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FMMBGX3_n01_cotylset2_05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25095155
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC380480.1516149232790154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG126150.023.4922715
CACCTTA209950.019.9817817
CTAGTTG83550.019.2834328
ACCTTAG217850.019.2728948
GGACACC80700.018.89583629
CCTTAGA232400.018.199879
TACTCTA99750.016.7052634
TAGAGTA254900.016.56633212
TCTAGTT111050.016.3101257
GACACCT94150.016.30636230
ACCTCTA100800.015.95023217
CTAATGC99400.015.89704321
CTCTAGT109450.015.7291016
GCTGACT81500.015.4936851
GTTACCT104050.015.41806814
TTAGAGT276200.015.27621811
CTTAGAG277950.015.20489110
CTAGCAG88800.014.9964438
GACTCTA95000.014.8894744
TCCACCT287000.014.7256115
Produced by FastQC (version 0.11.2)