FastQCFastQC Report
Thu 14 Sep 2017
H2FMMBGX3_n01_cotylset2_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FMMBGX3_n01_cotylset2_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20497127
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC462580.22568040877143417No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATC250710.12231470293373312No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC233260.11380131469156628No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTA224340.10944948528640137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG103450.026.1458025
CTAGTTG76550.022.8043388
TACTCTA80050.021.8074824
GGACACC75450.021.39931129
CACCTTA250200.021.1245677
ACCTTAG252200.020.8884438
CTCTAGT92150.020.2169116
CCTTAGA267750.019.68829
TCTAGTT95700.019.6472117
TTTTTCC273200.019.3599531
ACCTCTA87000.019.31307817
GACACCT87750.018.59632930
GTTACCT92150.018.45778514
TTTCCAC293550.018.4045853
TCCACCT293800.018.0014175
TAGAGTA295700.017.92062212
CTAATGC93450.017.9056221
TTTTCCA310100.017.8562242
TTCCACC309850.017.2693774
CCACCTT307150.017.2526996
Produced by FastQC (version 0.11.2)