FastQCFastQC Report
Thu 14 Sep 2017
H2FMMBGX3_n01_cotylset2_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FMMBGX3_n01_cotylset2_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21058111
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC715570.33980730750255805No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATC386060.18333078403851136No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC379940.18042454045379472No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTA332340.15782042368377677No Hit
ATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAA237320.11269766789623248No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCT236830.11246497845889405No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCA229720.1090886072354733No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCT218720.10386496680542714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG128500.031.4120755
CTAGTTG103750.029.0626458
TACTCTA111750.027.2909474
TCTAGTT116100.027.1003867
GGACACC108450.026.11743729
CTCTAGT120000.025.6734436
ACCTCTA120150.024.86824417
GTTACCT121550.024.60862414
TTTTTCC308300.024.5298791
CTAATGC123150.023.84168221
GACACCT121350.023.3979130
ATGCTGG128350.022.82085624
TACCTCT130550.022.80777216
TGTTACC134950.022.471613
CACCTTA339550.022.3426347
TTACCTC134800.022.31872415
TTTTCCA356850.022.1682932
ACCTTAG341800.022.155138
CCTCTAA136650.022.09267818
TGGACAC133400.021.4395528
Produced by FastQC (version 0.11.2)