FastQCFastQC Report
Thu 14 Sep 2017
H2FMMBGX3_n01_cotylset2_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FMMBGX3_n01_cotylset2_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21636317
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC321060.1483893954779827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG92450.020.114065
CACCTTA193150.018.5405037
ACCTTAG198500.018.0058298
CTAGTTG69300.016.9759868
CCTTAGA217050.016.5782389
GGACACC66750.015.71227929
TAGAGTA233000.015.56190312
TACTCTA75800.015.4293774
TTAGAGT249400.014.42785411
TCTAGTT94850.014.4037897
GTTACCT82050.014.33833414
CTTAGAG254700.014.15471810
TCCACCT255100.014.1461775
CTCTAGT92700.013.9934886
TTTTTCC257800.013.9584561
CCACCTT258050.013.9577216
ACCTCTA83100.013.82547917
CTAATGC86050.013.75223121
TTTCCAC270250.013.6723023
AGAGTAC272100.013.43987513
Produced by FastQC (version 0.11.2)