FastQCFastQC Report
Wed 13 Sep 2017
H2FMMBGX3_n01_cotylset2_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FMMBGX3_n01_cotylset2_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18002180
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG74350.016.2408055
CACCTTA87400.014.5263627
ACCTTAG92150.013.8896278
CGGGAAT75050.013.7454161
GCTGACT53600.013.131151
CCTTAGA98100.013.0823569
GACTCTA66300.012.0724374
TAGAGTA106950.011.99980512
CATGCCG59150.011.95699463
CGGTACC63150.011.6365214
GGTACCG61400.011.57511715
GTACCGA60850.011.56647216
TGCCGAG62900.011.51841565
CTAGCAG62250.011.4166838
ATGCCGA65450.010.96422264
TACCGAG69450.010.78009117
TCTAGCA126150.010.7753037
TACCCTA111100.010.744240
GTACAGC65750.010.70400851
ACCGAGC69100.010.58504718
Produced by FastQC (version 0.11.2)