FastQCFastQC Report
Sat 21 Apr 2018
H2FCFAFXY_n02_vm352.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FCFAFXY_n02_vm352.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10338648
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG130370.12609966022636615No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA76700.029.46150269
GGTGTAG143100.018.63583669
AGGTGTA145850.018.3552468
GAGGTGT196700.014.36443469
GTATAAT10750.013.1631171
GTATAGA12100.013.1206641
AGAGGTG253650.012.73063269
TAGAGAG429750.012.72396269
ATAGAGA531050.012.7134869
AATAGAG644200.012.3815869
AGAGAGG365550.012.37271269
TATAGAC18350.012.2228482
GAGAGGT285550.012.19040169
TTTATAC13800.012.0001853
GAATAGA780900.011.7338869
ATCTAGG23400.011.5043511
TATAATA14700.011.5020132
GTATATA15400.011.4296461
GTATTAA19400.011.2078471
TGTAGAT55150.011.19738269
Produced by FastQC (version 0.11.2)