FastQCFastQC Report
Sat 21 Apr 2018
H2FCFAFXY_n02_vm34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FCFAFXY_n02_vm34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15297400
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG182090.1190332997764326No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA118450.031.54318269
GGTGTAG219100.018.95816269
AGGTGTA225650.018.30047868
GAGGTGT307000.015.62027269
AGAGGTG409900.014.97304569
TGTAGAT79400.014.90341369
GAGAGGT492400.014.48920769
TAGAGAG758100.013.48395569
AGAGAGG631900.013.34881269
ATAGAGA925950.012.42941369
AATAGAG1118050.011.83358269
GTATATA26300.011.1543131
TATAAGG20250.010.9062392
GAATAGA1335150.010.66649669
GTATTAT32700.010.4488161
ATCTAGG30750.010.43800451
GTATTAA31200.010.0662211
TCTAGGA42600.09.9635832
GTACTAA25450.09.8995341
GTATTAG25650.09.8223441
Produced by FastQC (version 0.11.2)