FastQCFastQC Report
Sat 21 Apr 2018
H2FCFAFXY_n02_vm331.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FCFAFXY_n02_vm331.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12366776
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA73800.026.27208969
AGGTGTA124300.016.34758468
ATAGAGA508800.015.0190169
TAGAGAG396050.014.62565669
GGTGTAG143550.014.58814869
GAGAGGT253800.013.9602669
GAGGTGT171250.013.86029669
AATAGAG632100.013.67214469
AGAGAGG334050.013.41566969
AGAGGTG217950.012.15679269
GTATTAA27500.012.0483361
GAATAGA775200.011.79806169
GTATAAT17450.011.2737391
TATAGAC20850.011.0884142
GTATATA21400.010.9668941
GTATAGT23000.010.5040981
TACTATA23300.010.36675552
CGAATAG882650.010.33042168
GTATTAC28700.010.2217571
GTATTAT29200.010.1649251
Produced by FastQC (version 0.11.2)