FastQCFastQC Report
Sat 21 Apr 2018
H2FCFAFXY_n02_vm322.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FCFAFXY_n02_vm322.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15437470
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG173550.1124212711020653No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA141700.035.3270769
GGTGTAG270700.021.86950969
AGGTGTA292000.020.12038268
GAGGTGT394100.015.45945969
TGTAGAT78800.014.5352369
AGAGGTG498250.012.98959369
TAGAGAG859050.012.64632269
GAGAGGT583800.012.59891569
GTATATA21100.012.5952911
TATACCG18450.012.5282755
GTATAGA18300.012.4478051
ATAGAGA1046000.012.18358969
AATAGAG1267850.012.10066669
TATAGAC24700.011.8746832
AGAGAGG727850.011.84024269
GTATTAA27250.011.6525571
GAATAGA1536400.011.54841269
GTATTAT28850.011.3652171
GTATAAT17800.011.0523541
GTACTAA24100.011.0274131
Produced by FastQC (version 0.11.2)