FastQCFastQC Report
Sat 21 Apr 2018
H2FCFAFXY_n02_vm319.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FCFAFXY_n02_vm319.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10526371
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG239250.22728630788331514No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGAG174450.016.45415769
AGAGAGG150100.016.25032469
GAGAGGT100050.015.79325669
ATAGAGA223750.013.86181269
AATAGAG263450.011.72027568
AGAGGTG93300.010.79753269
GTATAGA16850.010.4456121
GAATAGA313650.09.86643367
GTATTAA30550.09.8281471
TATAGAC22300.09.7480752
GTGTAGA42950.09.71953569
GAGGTGT67500.09.35342469
TATACCG13450.09.2338955
CGAATAG340900.09.16905369
AGGTGTA49500.09.13017568
GTATAGT24200.09.1270031
GTTATAA12250.09.01524451
GCCGACG644850.08.99357369
TATACGG10900.08.8620935
CCGACGA573950.08.55971369
Produced by FastQC (version 0.11.2)