FastQCFastQC Report
Sat 21 Apr 2018
H2FCFAFXY_n02_vm312.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FCFAFXY_n02_vm312.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11698311
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG126690.10829768502478691No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA63900.019.81478769
AGGTGTA97700.015.00746968
GTATAAT17250.014.0053151
AATAGAG432300.013.84645169
GAATAGA551650.013.72761569
GAGAGGT187650.013.49496469
GAGGTGT133550.013.3558369
GTATTAG20900.013.2107531
ATAGAGA349750.013.11952769
GTATAGG17600.012.94240951
CGAATAG652150.012.74422869
TAGAGAG280050.012.22081969
GTATAGA17800.012.2153091
GGTGTAG124800.012.05302769
GTATAAG15200.011.8071111
AGAGAGG245750.011.75049569
GTATATA23850.011.7214281
AGAGGTG174750.011.62846369
TAAGGGT14050.011.5406684
TATAATA25350.011.4340422
Produced by FastQC (version 0.11.2)