FastQCFastQC Report
Sat 21 Apr 2018
H2FCFAFXY_n01_vm352.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FCFAFXY_n01_vm352.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10338648
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG87000.023.99133969
TGATCTC122300.019.43618869
ATGATCT180150.013.46292468
CTAGGCA520000.013.14978569
TATAGAC17700.013.0598682
GTATAAG12800.012.9396051
GTATTAA18050.012.8081781
TAGGCAT424950.012.80301369
ACTAGGC630850.012.62746469
TTATACG7650.012.6273694
GCATGAT242950.012.34017369
AGGCATG370750.012.25527369
CATGATC197150.012.21441767
GACTAGG766850.011.98961369
ATATAGA21600.011.8213671
TATTAAG10250.011.780982
GTATAGA12400.011.4091141
TTAAGGT10050.011.3282844
GGCATGA304200.011.18242669
GTATTAG16700.010.9508831
Produced by FastQC (version 0.11.2)