FastQCFastQC Report
Sat 21 Apr 2018
H2FCFAFXY_n01_vm34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FCFAFXY_n01_vm34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15297400
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG114200.029.12223469
GAATCTC185700.019.67423269
GGCGAAT367750.016.08885869
CGAATCT234450.015.86288868
AGGCGAA481050.014.70203469
ATCTCGT74750.014.630589569
GCGAATG64600.014.205708569
GCGAATC263350.014.17445367
TAAGGCG738250.013.77646469
AAGGCGA628750.013.43768769
CTAAGGC913600.012.61258669
ACTAAGG1103000.012.07016169
GTATTAG23750.011.1865521
GTATTAC28350.010.9536431
GACTAAG1307900.010.91515169
GTATAAA25000.010.9032561
GTATTAA29400.010.4450811
GTATTAT32150.010.1955761
TTATACT26400.010.0625084
GTATATG22050.09.8582791
Produced by FastQC (version 0.11.2)