FastQCFastQC Report
Sat 21 Apr 2018
H2FCFAFXY_n01_vm331.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FCFAFXY_n01_vm331.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12366776
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG59100.029.42095469
CTATCTC97850.019.0743769
CGGACTC492700.015.39771969
CCTATCT125950.014.87357568
ACTCCTA236800.014.83131969
GGACTCC401450.014.41170469
ACGGACT621950.013.8175869
CTCCTAT186400.013.41855769
GACTCCT330100.013.26263569
TCCTATC154400.012.6245269
GACGGAC759950.011.84411969
GTATTAT29200.011.5802621
GTATAAT19400.011.5607721
GTATAAA19050.010.68641951
GTATTAA24150.010.5727941
TATTATA28250.010.5029932
GTATTAC29000.010.3513371
AGACGGA893200.010.12353268
GTATTAG21400.09.9966181
TATAGAC22250.09.9239152
Produced by FastQC (version 0.11.2)