FastQCFastQC Report
Sat 21 Apr 2018
H2FCFAFXY_n01_vm322.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FCFAFXY_n01_vm322.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15437470
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTCG139850.030.836469
GCATCTC214550.022.17439869
AGCATCT293250.016.58812168
GAGCATC363850.014.21326867
ATCTCGT68400.013.76964269
TGAGCAT457250.012.86431969
GTATAGA18750.012.5137781
TCCTGAG871650.012.42410969
ATATAAT24700.012.2932451
CCTGAGC700250.012.29231169
CTGAGCA588550.012.26293669
CTCCTGA1063450.011.85731769
GAGCATG90950.011.53153169
TATAATA23050.011.5256512
GACTCCT1553000.011.40291569
ACTCCTG1369500.011.30846569
AGCATCG87750.011.12641969
TTAGACA22750.011.0703964
ATATAGA28100.010.9285991
GTATTAG22100.010.7730241
Produced by FastQC (version 0.11.2)