FastQCFastQC Report
Sat 21 Apr 2018
H2FCFAFXY_n01_vm319.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FCFAFXY_n01_vm319.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10526371
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG33800.018.06648643
CTCGTAT36650.016.75564239
GCCGTCT37850.016.49794446
AAGGGGG39700.015.12083869
TCGTATG42300.015.08851240
CGTATGC43150.014.55149841
GGCAGAA167900.014.52733269
AGGCAGA206650.013.99025869
TCTCGTA44950.013.43146538
GTATGCC47850.012.97799742
ATGCCGT49350.012.79325544
CCGTCTT50000.012.62694447
AAAGGGG46950.012.56543768
CAGGCAG247850.012.49984269
AGGGGGG25350.011.43184767
GCAGAAA141500.011.38616869
TGCCGTC54850.011.38463345
AATCTCG54050.011.29776736
GTATATA21400.011.2862831
GTATTAT30000.011.1562211
Produced by FastQC (version 0.11.2)