FastQCFastQC Report
Sat 21 Apr 2018
H2FCFAFXY_n01_vm312.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2FCFAFXY_n01_vm312.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11698311
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG66500.017.43132469
AGATCTC85900.015.58300869
TATAATA24250.015.5083152
GTATAAT18500.015.1077021
GACCGTA527550.014.13854869
GTATTAA25650.013.4523381
CCGTACT348450.013.02951269
GTATTAT26800.013.0038431
AGACCGT645300.012.83639969
GTATAGA18850.012.8136211
ACCGTAC469500.012.80045269
TACTAGA196150.012.73394669
ACTAGAT148550.012.70361769
CGTACTA268950.012.66071869
GTATTAG21100.012.5919621
GTATAAA17100.012.5106741
TAGATCT108350.012.44974868
TATAGAC24450.012.1358292
GTATTAC27850.012.0179861
GTATATA25100.011.6850431
Produced by FastQC (version 0.11.2)