FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11480254
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3039142.647275922640736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACT7500.015.8845425
GCGGACT6900.013.2032625
GACTGTA20950.011.5239598
CGGACTG31450.09.6887796
CCCGGAC5502.3775614E-58.2896074
TAGTCCG8951.0615622E-87.80717311
CGGACTA9101.1221164E-77.31278236
TACAGTC21350.07.030516625
CTAGTCC10754.220965E-86.826771310
GGACTCT11056.876871E-86.6539717
TGTAGAA41100.06.46038711
GGACTGT47100.06.3929527
GGACTAG12358.4999556E-86.2370567
TCGCCTG10252.49464E-45.46671769
TTCTACA42450.05.429352322
GTAGAAG55650.05.271610712
TAGGGAA126350.05.26633970
GATCTTC35050.05.18078118
CTGTAGA51800.05.127295510
GTAGGGA128400.05.100433369
Produced by FastQC (version 0.11.5)