FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n02_pIXSBP_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n02_pIXSBP_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2622761
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56080.21382047392042203No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG4550.040.06
GGACTGT5800.031.379317
CCGGACT3100.024.8387075
GACTGTA7800.022.4358988
CAGTCCG4750.020.6315827
ACTGTAG9100.020.09
GCGGACT2701.2369128E-1018.1481485
CGGACTA3153.6379788E-1217.7777796
CTGTAGA11450.016.20087410
CCCCTCG1400.003755630215.036
CGCGGAC1656.524078E-414.8484844
ACAGTCC6600.014.31818126
GCGTCGG2454.274789E-614.28571470
TCGGACT4453.6379788E-1214.1573035
TACAGTC7300.013.90410925
CTACAGT7500.013.53333424
TAGTCCG3203.5137418E-713.12511
GTAGAAG15800.012.18354512
TCTACAG9300.012.04301123
ACGGACT9050.011.2154695
Produced by FastQC (version 0.11.5)