FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n02_pIXHALO_SBP-bZIP53_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n02_pIXHALO_SBP-bZIP53_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3892340
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63370.16280694903322937No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG3950.023.0379546
GGACTGT7200.013.6110977
CAGTCCG5153.6379788E-1212.91260927
GACTGTA7600.012.8947258
GCGGACT3802.801682E-611.0526215
CGGACTA4301.0679214E-610.5813866
ACAGTCC6452.5956979E-99.76743326
CCGGACT4703.3365613E-69.6808415
TCGGACT5401.9771487E-69.0740655
ACTGTAG9901.8189894E-128.8383759
TACAGTC8101.5879777E-98.64196725
TAGTCCG3800.0038442288.28946611
GGACTAG6754.692478E-67.7777717
AGGGGGG7907.005128E-77.53202531
TTGCGCT4200.0094345297.50008962
CTGTAGA13353.6379788E-127.34081710
CTACAGT9754.9825758E-87.179480624
GTGTTAA65100.06.827950570
TCGTGTT21000.06.666660370
GAGTGTT77550.06.634423370
Produced by FastQC (version 0.11.5)