FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n02_WRKY18_col_B_MM9430_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n02_WRKY18_col_B_MM9430_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5961815
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60930.10220042050952605No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG5250.017.9999756
AGGGGGG5100.015.7856171
CAGTCCG5900.014.23726927
GGGCGCC2300.00789530410.65215915
GGACTGT9000.010.4999877
CGATGCG8403.6379788E-129.58332168
GCGATGC9454.5474735E-118.51850867
GTAGGCT23200.08.44826570
TGTAGGC28700.08.1707269
CCTACCG5151.021735E-48.15532956
GCGTCCT6907.3809315E-78.115931570
TAGGCTA23400.07.628195370
GGGGGCC9756.2736945E-97.538451759
ACTGTAG14050.07.47339
GAGTGTA46300.07.332603570
GGCCGGC10755.4351403E-97.162781262
ACAGTCC11301.9717845E-97.12388426
CTACAGT13352.5465852E-117.07864324
GATGCGT10907.177732E-97.064210469
ACGGCCG6602.3403612E-46.8939370
Produced by FastQC (version 0.11.5)