FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n02_SG163_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n02_SG163_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3076936
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG140690.45724057958956577No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG5850.032.3076176
GGACTGT7000.026.9999357
GACTGTA7350.022.857098
TCGGACT5250.019.9999525
CAGTCCG6100.017.78684427
TGTGCCG9450.016.66662870
ACTGTAG10200.015.7842789
CGGACTA2906.2354957E-915.6896196
ACGGACT10000.013.6499685
ACAGTCC8450.013.25440726
CTGTAGA12900.012.75190810
CTACAGT8750.011.99997124
GCGTCGG10850.011.93545570
TACAGTC8450.011.59760625
GACGGAC2903.2481167E-410.8620434
GCGGACT2903.2481167E-410.8620435
GCCCGGT2300.00789390110.65214934
CGGAAGC17150.010.6122270
CGGGACG2350.00925849310.42550835
AACGGAC4753.4322875E-710.3157654
Produced by FastQC (version 0.11.5)